Constance Knightover 3 years ago
How is human parainfluenza virus (HPIV) isolated?
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over 3 years ago
Isolation of HPIV Primary monkey kidney (PMK) cells of rhesus, African green monkeys and cynomologus, allow best growth of HPIV. Other cells for better growth of HPIV include LLC-MK2. For isolation of HPIV, add 2-3 mg/ml trypsin to the maintenance medium of LLC-MK2 cells. Protease plays important role in favoring the replication of primary cell cultures.
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