Zunaira saleh1 day ago

Dentin disk barrier test method.

A dentin disk is used as a barrier in cytotoxicity tests that attempt to predict the toxicity of materials placed on dentin in vivo. The material is placed on one side (A) of the dentin disk (B) in the device used to hold the dentin disk. Collection fluid (cell culture medium or saline) is on the other side of the disk (C). Cells can also be grown on the collection side. Components of the material may diffuse through the dentin and the effect of the medium on cell metabolism can then be measured. To assess the rate of diffusion, the collection fluid can be circulated into and out of the collection chamber (C).