Loop-Mediated Isothermal Amplification • Type of reaction: Isothermal reaction • Temperature: Isothermal temperature (60-65 C) • Economy: Does not require expensive thermocycler i.e. economical • Detection limit: Greater detection limit • Amplification limit: Higher amplification specificity • Visual detection: Visualization of DNA through eyes, turbidimeter, and gel electrophoresis • Number of primers: 4 • Analysis time: 45 mins • Ease of use: Easy to use • Sample inhibitor sensitivity: Insensitive • Knowledge of method: Little known • Clinical evaluation: Going on Polymerase Chain Reaction • Type of reaction: Cycle reaction • Temperature: Variable temperature i.e. denaturation (95 C), annealing (50-60 C), polymerization (72 C) • Economy: Requires expensive thermocycler i.e. not economical • Detection limit: Lower detection limit • Amplification limit: Lower amplification specificity • Visual detection: Visualization of gel electrophoresis • Number of primers: 2 • Analysis time: 6-8 h • Ease of use: requires trained staff • Sample inhibitor sensitivity: Sensitive • Knowledge of method: Well-known • Clinical evaluation: Clinically proven