MEDizzy - Increase or decrease in ________

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metabolism - Set 25

Increase or decrease in _________ leads to protonation or deprotonation of bases which leads to melting of DNA in turn isolating the enzyme after centrifugation.

ExplanationProtons are responsible for maintain the biologically native structure of enzymes and nucleic acids. Hence increase or decrease in pH results in protonation or deprotonation of bases which leads to denaturation if DNA. The resultant extract in then centrifuged to isolate enzymes. The main limitation of this technique is the loss of enzymes due to denaturation.