MEDizzy - You wanted to isolate active RNA polymerase (with a part of

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metabolism - Set 17

You wanted to isolate active RNA polymerase (with a part of the transcript associated with it ) from cellular mix. For this, you perform an immunoprecipitation with anti sigma antibody. Then you check the precipitate. What will you observe?

ExplanationSigma factor dissociates after initiation so anti sigma antibody will only pull down RNA polymerase prior to promoter clearance. Thus, the polymerases so obtained have no transcript associated.