UV shadowing is used at times for visualising the DNA. Which of the statements is correct for it?
ExplanationIn this method, the DNA bands won’t fluoresce but will absorb the UV light. This leads to the formation of a non-fluorescent shadow on the screen. It is suitable for visualising single stranded species because the mode of action is not intercalating as in the case of use of ethidium bromide. It is preferred over it because ethidium bromide is often difficult to remove.
