Hot-start PCR is a modification of PCR. Which of the following is not corresponding to it?
ExplanationHot-start PCR is a modification which is used in order to overcome the problem of the extension before the maximum temperature of first cycle is reached. Hence, polymerase is added only after the maximum temperature for first cycle is reached. Hence, there are more specific products which are generated because proper annealing of primers has taken place. It is suitable for a small number of samples but not for a large number of samples.
