With respect to Sanger’s enzymatic method of sequencing pick the odd one out.
ExplanationIn the reaction system for DNA replication, at least one of the four deoxyribonucleotides is radioactive in order to permit the autoradiographic development of bands after gel electrophoresis. A small primer sequence with a free 3’ – OH group must be provided with the template strand of DNA replication to proceed, since a free 3’ – OH is absolutely essential for the larger subunit of DNA polymerase I to catalyze DNA replication.
