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USMLE
Basic Medicine - Set 19
Which of the following enzymes is responsible for the digestion of the RNA strand in the RNA-DNA hybrid?
Explanation
ExplanationWhen making a cDNA library the DNA copy is made from an RNA template thus leading to the formation of the RNA-DNA hybrid. Thus for the production of the chimera molecule the RNA template needs to be hydrolysed which is provided by RNase H enzyme whereas RNase A enzyme removes RNA primer from the in case of replicating the lagging strand.
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