. A, Enamel specimens preparation involved the removal of a portion of superficial tissue without exposing the underlying dentin. B, Tooth prepared for dentin test: the occlusal third was removed with a diamond disc, creating a flat surface.C, Resin build-up over the enamel and the dentin surface. D, Cutting of the tooth along the X- and Y-axis and the resulting sticks. E, Procedure for the preparation of hourglassshaped specimens: the bonded tooth is sectioned in multiple slabs. On each slab the narrowest cross section is created at the interface by trimming with a bur.