MEDizzy
MEDizzy
Zunaira saleh
Zunaira salehabout 17 hours ago
Agar overlay method

Agar overlay method

. The agar overlay method has been used to evaluate the cytotoxicity of dental materials. The cell layer, which has been previously stained with neutral red (NR), is covered with a thin layer of agar (A). Samples are placed on top of the agar for a time. If the material is cytotoxic, it will injure the cells and the neutral red will be released, leaving a zone of inhibition.

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