MŐH!Talmost 5 years ago

BIURET TEST

Biuret Reaction: Cupric ions in an alkaline medium form a violet color with peptide bond nitrogen (Schiff, 1896). This needs a minimum of two peptide bonds, and so individual amino acids and di-peptides will not answer this test. This reaction can be used for quantitative estimation also. The name is derived from the compound biuret (NH2—CO—NH—CO—NH2), a condensation product of two urea molecules, which also gives a positive color test. Magnesium and ammonium sulphates interfere with this reaction