This is the first demonstration of whole heart DTI producing 3D tractograms of the entire LV using free breathing, of either the short-axis or 4-chamber views, in under 15 minutes. This clinically feasible protocol could facilitate the translation of whole heart DTI to patients with heart failure and other disease states. The characterization of myocardial microstructure using DTI in the clinical setting could provide important insights into disease pathogenesis and treatment.